Greyfriars Kirk, today Greyfriars Tolbooth & Highland Kirk, is a parish kirk (church) of the Church of Scotland in central Edinburgh, Scotland. The kirk stands on the site of a pre-Reformation establishment of the Franciscan order, the "Grey Friars".

It is one of the oldest surviving buildings built outside the Old Town of Edinburgh, having been begun in 1602 and completed circa 1620. It sits to the south of the Grassmarket. Greyfriars Kirk has an important place in the history of the Scottish Covenanters. In 1638 the National Covenant was presented and signed in front of the pulpit. In 1679, some 1,200 Covenanters were imprisoned in the Kirkyard pending trial.

For many, the graveyard is associated with Greyfriars Bobby, the loyal dog who guarded his master's grave. The kirkyard is reputedly haunted by the restless spirit of the infamous 'Bloody' Sir George Mackenzie, a former Lord Advocate, which is said to cause bruising and minor cuts and grazes on those who come into contact with it.